Table 2
Loci used in analysis of lake whitefish (Coregonus clupeaformis), their PCR conditions, fluorescent labels used, and reference for each locus.
| Multiplex/PCR Cycle | Locus | MgCl2 (mM) | Concentration primer (μM) | Label | Size range (base pairs) | References |
|---|---|---|---|---|---|---|
| 1a | Bwf1 | 1.25 | 0.1 | 56FAM | 190–268 | Patton et al., 1997 |
| 2b | Bwf2 | 1.5 | 0.15 | 56FAM | 139–167 | Patton et al., 1997 |
| 1a | C2-157 | 1.25 | 0.15 | 5HEX | 126–186 | Turgeon et al., 1999 |
| 2b | Cocl23 | 1.5 | 0.3 | 56FAM | 245–287 | Bernatchez, 1996 |
| 3c | CoclLav4 | 2 | 0.2 | 5HEX | 139–159 | Rogers et al., 2004 |
| 4d | CoclLav45 | 1.25 | 0.08 | 56FAM | 236–258 | Rogers et al., 2004 |
| 4d | CoclLav52 | 1.25 | 0.1 | 5HEX | 94–156 | Rogers et al., 2004 |
| 5c | CoclLav6 | 1.4 | 0.1 | 5HEX | 123–163 | Rogers et al., 2004 |
| 3c | CoclLav68 | 2 | 0.1 | NED | 167–183 | Rogers et al., 2004 |
| 6e | CoclLav8 | 1.5 | 0.2 | 5HEX | 199–251 | Rogers et al., 2004 |
| 7e | Sfo23 | 2 | 0.25 | 56FAM | 147–207 | Angers et al., 1995 |
| 8e | Sfo8* | 2 | 0.25 | NED | 205–259 | Angers et al., 1995 |
95°C for 3 min; 15 cycles of 94°C for 1 min, 60°C for 45 s, 72°C for 10 s, then 20 cycles of 94°C for 30 s, 60°C for 30 s, 72° for 10 s, and a final elongation at 72°C for 5 min.
95°C for 3 min; 15 cycles of 94°C for 1 min, 55°C for 45 s, 72°C for 10 s, then 20 cycles of 94°C for 30 s, 55°C for 30 s, 72° for 10 s, then a final elongation at 72°C for 5 min.
95°C for 3 min; 35 cycles of 95°C for 1 min, 60°C for 30 s, 72°C for 1 min, then a final elongation at 72°C for 5 min.
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