Open Access
Table 5
Results of in silico tests for the 16S and 23S primers including adapters from the sequencing platform: ΔTm is the melting temperature difference between forward and reverse; Oligonucleotide structure warnings.
| Gene marker | Primer pairs | Δ Tm (°C) | Oligonucleotide structure warnings |
|---|---|---|---|
| 16S | CYA359F/CYA781R | 1.1 | 1 Heterodimer |
| ECLA16S_F1/ECLA16S_R1 | 2 | 1 Heterodimer | |
| 23S | p23SrV_f1/p23SrV_r1 | 0.6 | 1 Heterodimer |
| ECLA23S_F1/ECLA23S_R1 | 0.5 | none | |
| ECLA23S_F2/ECLA23S_R2 | 1 | none |
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