Open Access

Table 5

Results of in silico tests for the 16S and 23S primers including adapters from the sequencing platform: ΔTm is the melting temperature difference between forward and reverse; Oligonucleotide structure warnings.

Gene marker Primer pairs Δ Tm (°C) Oligonucleotide structure warnings
16S CYA359F/CYA781R 1.1 1 Heterodimer
  ECLA16S_F1/ECLA16S_R1 2 1 Heterodimer
23S p23SrV_f1/p23SrV_r1 0.6 1 Heterodimer
  ECLA23S_F1/ECLA23S_R1 0.5 none
  ECLA23S_F2/ECLA23S_R2 1 none

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.