Table 4

Results of in silico tests for the 16S and 23S primers. ΔT_m: melting temperature difference between forward and reverse. Oligonucleotide structure warnings: warnings obtained with OligoAnalyzer. In silico PCR output: number of sequences that perfectly match with primers (in brackets) followed by the number of sequences likely to be amplified out of the total number of reference sequences. Estimated resolution: percentage of species with strictly different barcodes (in brackets are given the exact numbers of species with different barcodes out of the total number of species). Estimated specificity: for 16S it is the proportion of phytoplankton species out of the proportion of heterotrophic bacteria amplified in silico; for 23S it is given as the result of in silico PCR done on heterotrophic bacteria sequences (i.e. « in silico PCR output »).