Open Access

Table 1

Primer pairs tested in silico. Combinations not tested before this study are indicated with a star (*).

Gene marker Primer pairs Targeted region Mean amplicon size (pb) References for primers definition References for primers tests
16S 341F/805R v3-v4 443 Herlemann et al. (2011) Eiler et al. (2013); Bennke et al. (2018)
  CYA359F/CYA781Rd v3-v4 425 Nübel et al. (1997) Costa et al. (2016); Ivanova et al. (2019)
  CYA359F/805R v3-v4 425 Nübel et al. (1997) (F); Herlemann et al. (2011) (R) *
  PLA491F/805R v4 315 Füller et al. (2006) (F); Herlemann et al. (2011) (R) *
  515F/926R v4-v5 413 Caporaso et al. (2011), modified by Parada et al., 2016 Parada et al. 2016; Watanabe et al. (2001)
  CYA781Rd/E1115R v5-v6 336 Nübel et al. (1997) (F); Reysenbach & Pace, 1995 (R) *
  ECLA16S_F1/ECLA16S_R1 v5-v6 386 This study *
  E939F/OXY1313R v6-v7 417 Rudi et al. (1997); West et al. (2001) *
23S p23SrV_f1/p23SrV_r1 v5 408 Sherwood & Presting, 2007 Craine et al. (2018); Brown et al. (2022)
  A23SrV_F1/A23SrV_R1 v5 411 Yoon et al. (2016) *
  A23SrV_F2/A23SrV_R2 v5 405 Yoon et al. (2016) *
  ECLA23S_F1/ECLA23S_R1 v5 402 This study *
  ECLA23S_F2/ECLA23S_R2 v5 408 This study *

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