Open Access
Table 1
Primer pairs tested in silico. Combinations not tested before this study are indicated with a star (*).
| Gene marker | Primer pairs | Targeted region | Mean amplicon size (pb) | References for primers definition | References for primers tests |
|---|---|---|---|---|---|
| 16S | 341F/805R | v3-v4 | 443 | Herlemann et al. (2011) | Eiler et al. (2013); Bennke et al. (2018) |
| CYA359F/CYA781Rd | v3-v4 | 425 | Nübel et al. (1997) | Costa et al. (2016); Ivanova et al. (2019) | |
| CYA359F/805R | v3-v4 | 425 | Nübel et al. (1997) (F); Herlemann et al. (2011) (R) | * | |
| PLA491F/805R | v4 | 315 | Füller et al. (2006) (F); Herlemann et al. (2011) (R) | * | |
| 515F/926R | v4-v5 | 413 | Caporaso et al. (2011), modified by Parada et al., 2016 | Parada et al. 2016; Watanabe et al. (2001) | |
| CYA781Rd/E1115R | v5-v6 | 336 | Nübel et al. (1997) (F); Reysenbach & Pace, 1995 (R) | * | |
| ECLA16S_F1/ECLA16S_R1 | v5-v6 | 386 | This study | * | |
| E939F/OXY1313R | v6-v7 | 417 | Rudi et al. (1997); West et al. (2001) | * | |
| 23S | p23SrV_f1/p23SrV_r1 | v5 | 408 | Sherwood & Presting, 2007 | Craine et al. (2018); Brown et al. (2022) |
| A23SrV_F1/A23SrV_R1 | v5 | 411 | Yoon et al. (2016) | * | |
| A23SrV_F2/A23SrV_R2 | v5 | 405 | Yoon et al. (2016) | * | |
| ECLA23S_F1/ECLA23S_R1 | v5 | 402 | This study | * | |
| ECLA23S_F2/ECLA23S_R2 | v5 | 408 | This study | * |
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