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Assigning taxonomy and traits to DNA sequences of river diatoms in a region with limited taxonomic knowledge using the updated and annotated reference library Diat.barcode

Maria Mercedes Nicolosi Gelis, Raphaëlle Barry-Martinet, Jean-François Briand, Teofana Chonova, Joaquín Cochero, Gilles Gassiole, Maria Kahlert, Balasubramanian Karthick, François Keck, Martyn Kelly et al. (12 more)

We present Diat.barcode v12, an updated and expert-curated diatom reference library that incorporates newly published rbcL sequences annotated with ecological and biological traits. Using 320 river samples from France and French Guyana, we show that direct trait assignment can complement taxonomy by providing ecological interpretability in regions with limited taxonomic coverage, supporting the use of traits as an additional tool for biomonitoring.

Int. J. Lim., 61 (2025) 10

Published online: 25 November 2025

DOI: https://doi.org/10.1051/limn/2025009

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