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Table 3

Inhibitory effect of the 11 agrochemicals and the mixtures/combinations tested on three species of microalgae. Brain–Cousens model was used for the calculation of the 50% inhibition concentration (IC50) value in molarity for the 11 agrochemicals and as a fraction of the RCV for the mixtures and combinations, the associated determination coefficient (R 2) and the standard deviation (SD). NI, IC50 could not be determined within the concentration range. A letter indicates a significant difference between the mean values (ANOVA, Pr (>F) < 0.05; Tuckey HSD test, P. adj < 0.05) of the mixtures/combinations.

  D. subspicatus N. palea N. pelliculosa
  IC50 ± SD (nM) R 2 IC50 ± SD (nM) R 2 IC50 ± SD (nM) R 2
ACLO NI   NI   NI  
ATRA 1 178 ± 255 0.93 1 987 ± 24 0.97 NI  
CLOM NI   NI   NI  
FENO 14 884 ± 3 564 0.84 NI   NI  
IMAZ NI   NI   NI  
IODO 514 ± 80 0.99 NI   NI  
ISOX NI   NI   NI  
META 900 ± 174 0.99 NI   NI  
SMET NI   NI   NI  
DEA 30 349 ± 5 041 0.99 49 855 ± 218 0.93 NI  
BE 21 946 ± 1 0.94 NI   18 913 ± 733 0.98
  IC50 ± SD (fraction of RCV)   IC50 ± SD (fraction of RCV)   IC50 ± SD (fraction of RCV)  
MixAll 3.75a ± 0.30 0.99 NI   6.41 ± 0.33 0.99
MixDs 1.26a,b ± 0.04 0.99 NI   NI  
MixDsw/oIODO 4.41b ± 0.22 0.99 NI   NI  
IODO 1.83a,b,c ± 0.23 0.99 NI   NI  
IODO+BE1 1.87 ± 0.38 0.98 NT   NT  
IODO+BE2 4.67c ± 0.93 0.93 NT   NT  

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