Which barcode to decipher freshwater microalgal assemblages? Tests on mock communities

Alexis Canino, Clarisse Lemonnier, Benjamin Alric, Agnès Bouchez, Isabelle Domaizon, Christophe Laplace-Treyture and Frédéric Rimet

Microalgae taxa can be identified with short DNA barcodes. Eight primer pairs for 16S and five for 23S were tested in silico and with mock communities including cyanobacteria and eukaryotic microalgae. We conclude that the 23S primer pair ECLA23S_F1/ECLA23S_R1 was the best candidate to decipher freshwater phytoplankton communities.

Int. J. Lim., 59 (2023) 8
DOI: 10.1051/limn/2023008

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